The Phasefocus Livecyte Kinetic Cytometer

The Livecyte kinetic cytometer produces exceptionally high contrast time-lapse videos using Phasefocus’s patented Ptychographic Quantitative Phase Imaging (QPI) technology for a range of label-free assays with or without up to seven channels of complementary fluorescence.

Automated single-cell tracking of even the most sensitive cells quickly reveals subtle phenotypic differences in unperturbed cell populations.

Easy-to-use dashboards present coherent and concise results from up to 96 wells at a time whilst retaining the ability to investigate individual cell behaviour and outlying characteristics.

Livecyte Kinetic Cytometer Applications 

Explore the tabs below to learn more about the various Livecyte applications including videos, application notes and more. 

Cell Growth and Proliferation

  • Livecyte measures individual cell growth independently from proliferation
  • Cell growth is more complex than just cells multiplying
  • They can increase in size without dividing, they can divide asymmetrically, they can grow to a certain size then stop. All these aspects of cell growth are lost with most live cell assays. Not with Livecyte

Wound Healing

  • Directly measure cell motility with individual cells
  • Separate out migration from proliferation in a single experiment
  • Observe differences between leading edge and following cells
  • Correlate motility to morphological differences

Read the Application Note 'Single Cell Scratch Assay' from Phasefocus here.

Cell Motility

  • Livecyte automatically tracks cells, even in a 96-well plate
  • Just seed your cells, monitor with Livecyte and a full range of cell migration parameters will be generated
  • Compare the speed, directionality, confinement and heterogeneity of different populations

Oncology

  • Directly measure mitotic time with individual cells
  • Measure random and chemotactic migration
  • Identify and characterise differences in subpopulations in heterogenous primary cultures
  • Correlate motility to morphological differences
  • Generate statistically valid results from multiple repeats in 96-wells

Application notes

Toxicology

  • True label-free cell count, not just confluence
  • Monitor dry mass to automatically determine viability and cell death
  • Statistically robust results from multiple repeats in 96-well plates
  • Generate label-free dose-response curves
  • Identify dynamic toxic responses missed by other methods

Application notes

Angiogenesis

  • Label-free timelapse tube formation assays
  • High-resolution large fields-of-view with no stitching
  • Perform assays on Matrigel and standard plates
  • Automated post-acquisition focus ensures consistent image quality for every well
  • Simple, yet powerful analysis of tube formation metrics with Application Dashboard outputs

Stem Cells

  • Identify morphological phenotypes
  • Watch and quantify cell differentiation
  • Monitor stem cell populations, for many days, without perturbing cell behaviour
  • Image through plastic and coated plates
  • Image on extracellular matrices
  • Cells are still viable after imaging

Livecyte Kinetic Cytometer Videos

Livecyte: Multiple Assays In Every Experiment

See how Livecyte maximises every experiment, saving time and conserving precious cells.

This short 80-second video illustrates how proliferation, morphology and motility metrics are automatically generated and compared, down to single-cell level, in every Livecyte assay.

Livecyte: Fluorescence Gating

Want to analyse mixed cell populations and co-cultures?

This short 90-second video shows how Livecyte combines both label-free and fluorescence imaging to easily identify distinct sub-populations of cells and compare behaviour within mixed populations, by gating on fluorescence labels.

Livecyte: Explore new cell relationships

Go from plate, to population, to individual cell using the "Explore" functionality, where intuitive visual data representations will let you interrogate your cells, gate sub-populations and investigate the links between data-points and images.

Livecyte: Long Term - Large field of view (FOV)

With Livecyte, track your cells for periods of days or weeks, without the need for image stitching to track every cell through its lifecycle.

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