Logi-CHROM

HPLC Series

The Logi-CHROM pump is compact yet powerful featuring a high quality HPLC pumping system. Choose between 3 configurations:

• A 2 × 2 binary high pressure gradient (HPG) for high accuracy blending of up to two eluents from two selectable solvents each.
• A low pressure gradient (LPG) for reliable blending of up to four eluents.
• A cost-effective isocratic version for simple analyses.

Choice of pump heads available including:

• Maximum flow rate of 10 ml/min and 700 bar backpressure.
• Maximum flow rate of 5 ml/min and 1000 bar backpressure.

Whatever your separation requirements the Logi-CHROM system has a high quality pump set up available.

A wide range of detectors are available including:

• Radio chemical detector (Beta-RAM).
• UV detectors.
• Multi wavelength detectors.
• Diode Array Detectors.
• Refractive Index Detectors with intelligent temperature control.
• Electro Chemical detectors.

These detectors can be fitted with a range of flow cells to meet all analytical requirements.

The LogiCHROM autosampler is designed to give maximum efficiency and accuracy with superb sample integrity. It features the integrated intermediate loop decompression valve (ILD) which reduces sample dilution and increases measurement reproducibility.

• Up to 1 240 bar (700 bar).
• Cooling/heating option (4 – 40 °C).
• 0.1 μl – 10 000 µl (depending on configuration) sample injection volume.
• Up to 768 samples (microtiter plates) or 108 standard vials.
• Intermediate Loop Decompression – ILD.

The Logi-CHROM system offers a forced air column thermostat capable of heating or cooling from 5 to 85°C. The powerful fan and robust peltier element prevent a column from overheating while keeping it at a very stable temperature.

• Temperature range 5 – 85°C.
• Heating/cooling rate 2°C/min.
• Temperature accuracy ± 0.2°C.
• Temperature stability ± 0.1°C.
• Optional pre-column eluent tempering.
• Up to 8 columns with 350 mm max. length.
• Max. 16 mm inner diameter.
• Column switching option with ASM 2.1L.

Stable temperature has an immense positive influence on retention time reproducibility and the Logi-CHROM system offers analysts that temperature stable environment.

The Logi-CHROM ET 210 eluent tray enables you to blanket all your LC mobile phases with an inert Helium gas atmosphere in a user-friendly and easy way.

An atmosphere of inert gas in the solvent bottle prevents diffusion of air into the mobile phase and will keep it free of CO2 and O2. Especially in carbohydrate analysis using Anion Exchange Chromatography (HPAEC) based on separation with strong alkaline eluents, dissolved CO2 can be problematic. Under these circumstances (pH > 12), CO2-3 ions can be easily formed in the mobile phase, causing variations in retention times, decrease in column selectivity and loss in resolution.

Keeping the mobile phase free of carbonate is one of the key factors towards reproducible carbohydrate analyses via Anion-Exchange Chromatography. The ET 210 is the perfect choice to keep your mobile phase ‘carbonate-free’.

• Sparging followed by blanketing for minimal He consumption.
• Guaranteed carbonate free assuring highest reproducibility.
• Easy in use, all parts provided for direct installation.
• Mobile phase preparation and use under optimised conditions.

Synthesising and validating [18-F]PARPi

A Logi-CHROM has recently been installed at Essen University Hospital’s Clinic for Nuclear Medicine. The system was procured to complement the clinic’s existing suite of HPLC instruments to increase capacity for a growing team of radiochemists.

Anna Pacelli carries out postdoctoral research at the radiopharmacy into the automation and validation of radiopharmaceuticals for clinical use, specifically [18F]PARPi which was originally developed in the United States but had no automation and scale-up. Anna uses the Logi-CHROM with a Flow-RAM radio-HPLC detector for synthesis and optimises the radiolabelling and quality control, explaining why it was chosen over other HPLC solutions.

Click the button below to find out what Anna had to say.

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Developing an alternative PET quantification research methodology

The Logi-CHROM, along with the Posi-RAM, are being used to study arterial input function (AIF) at INRAE, France’s National Research Institute for Agriculture, Food, and Environment. Director of Research Professor Charles-Henri Malbert has developed an integrated autonomous system for PET quantification in miniature pigs as an alternative to non-human primates (NHP) before progressing to clinical trials for drug evaluation in humans.

In Charles’ integrated system, blood is circulated through an arterial catheter inserted in a femoral artery, passed through the Posi-RAM, and then returned through a saphenous vein. Blood flow in the external transit loop is controlled using a transit time flow meter and AI-controlled pump. Simultaneously, the radioactivity of the PET metabolites in the blood is measured using the Posi-RAM and recorded. Finally, blood is converted to plasma for radio-HPLC at known intervals for discrete sampling. The Logi-Chrom's autosampler is used for the automatic injection of plasma samples into the HPLC set-up.

Click the button below to find out more about Charles' research.

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