Beta-RAM 6

Radio Flow Detector for HPLC

IRIS technology allows users to optimize sensitivity and resolution by making a range of cell volumes available dynamically. Unlike traditional radio flow detectors for HPLC, which rely on a fixed flow cell volume, the IRIS technology gives a wide range of cell volumes without the need to change the flow cell.

The compact and integrated liquid manifold handles the liquid distribution and mixing with scintillation cocktail. The manifold is designed to reduce dead volume to the minimum, ensuring excellent peak shape and resolution.

The stop flow mode allows the user to achieve superb levels of sensitivity previously unavailable with on-line counting methods. By controlling both the Beta-RAM and HPLC system, the user can define a fraction time and counting time therefore eliminating the labour intensive, time consuming process of fraction collecting.

All parameters relative to flow cell type and serial number are seamlessly identified in the Laura software.  Cell ID allows for automatic calculation of eluate residence time and other counting units such as cpm and dpm.

Active Counting Mode allows the system to monitor and adjust run conditions in real-time, dynamically applying the most appropriate settings. This gives on-line detection limits previously only achieved off-line, as well as improved resolution and peak definition.

The Beta-RAM offers the versatility of either homogeneous counting by mixing with liquid scintillator via the accurate built-in pump or heterogeneous counting with a range of pre-packed solid scintillator flow cells.

The latest liquid chromatography technologies require dead volumes, connections and flow control of the scintillation cocktail to be finely managed. The parameters on the Model 6 are optimized to give superb performance with even the most rigorous LC demands.

The Beta-RAM can be easily stacked with HPLC systems; specifically, it has been designed to integrate with the LabLogic Logi-CHROM HPLC stack.

The Beta-RAM is controlled by LabLogic’s Laura software, which has been recognised as the industry standard radiochromatography software package for over 30 years. It features connectivity to the world’s leading HPLC systems and impressive data analysis.

With over 30 years of development and used by thousands of researchers worldwide the Beta-RAM radio flow detector for HPLC, coupled with the industry standard Laura software, continues to lead the way for radiochromatographers.

Stop Flow in action: An alternative to fraction collection

Senior Chemist Phil Lamond (inset) is using the Beta-RAM in studies for Environmental Risk Assessments at Fera's Sand Hutton site on the York Biotech Campus.

The Beta-RAM is being used by Fera Science for eFate studies. Located on the York Biotech Campus, it is a joint public-private research organisation for environmental management, conservation, and agriculture that adheres to guidelines defined by the Organisation for Economic Co-operation and Development (OECD) for the testing of chemicals in soil, sediment, and water. Fera performs Environmental Risk Assessments (ERA) for companies introducing new chemicals such as pesticides and biocides. Compounds are radiolabelled with ¹⁴C or ³H and degradation profiles mapped out over predetermined timepoints using chromatography, with studies lasting up to 90 and 220 days.

The Beta-RAM’s innovative Stop Flow measurement protocol increases the residence time of the analytes in the Beta-RAM’s flow cell, taking a snapshot of the sample at regular intervals. This increases the sensitivity of the radioactivity detector and improves the signal-to-noise ratio. LabLogic's HPLC system, the Logi-CHROM, fully supports Stop Flow functionality and is stackable with the Beta-RAM 6.

You can find out what Senior Chemist Phil Lamond had to say about Stop Flow by clicking the button below to find out more.

READ MORE

The experienced user described how when comparing the Beta-RAM 6 to the Perkin Elmer FSA, the Beta-RAM 6 is the clear winner. Read in more detail here.

The German chemistry giant chose the Beta-RAM 5 radio detector to be used at the BASF Agricultural Products Division at Research Triangle Park, North Carolina. Senior Research Associate, Dr Huns Nejad, explains in more details here.

The university chose the Beta-RAM for the study of phosphoinositides, a family of phospholipids that regulate many cellular processes. Dr David R Jones, an associate scientist in the Institute’s Inositide Laboratory, explains the work in more detail here.