Hidex Sense

Multi-Mode Microplate Reader

Microplate radiometric assays

The Hidex Sense Beta is a high-performance microplate liquid scintillation counter for beta and gamma counting. In addition, it has high sensitivity luminescence. This microplate counter supports all common radioactive assays and plate types such as cocktail based assays, filter plates, filtermats utilising the Hidex Filtermat Adapter, solid scintillators (e.g. LumaPlate®, FlashPlates®, MeltiLex®) and Scintillation Proximity Assays.

Multimode reader with radiometric detection

The Hidex Sense Beta Plus is an exceptionally versatile and compact high-performance microplate reader, combining all the technologies of the Hidex Sense multimode reader with the radioactive technologies of the Hidex Sense Beta. The Hidex Sense Beta Plus features all common non-radioactive detection technologies, high-sensitivity luminescence, and in addition liquid scintillation, beta, and gamma counting. It is the only microplate reader commercially available for both radioactive and non-radioactive technologies.

Absorbance spectral readout is obtained with a high sensitivity grating CCD spectrograph, scanning the complete sample spectrum from UV to IR in less than a second per well – or simultaneously up to 10 discrete wavelengths in one measurement.

An automatic filter storage with 32 filter positions used for either excitation or emission offers full wavelength coverage without a need for manual filter handling. This makes the instrument ideal for multiple users.

Uses optical filters for fluorescence and luminescence, which is recognised as the most sensitive. In addition, top and bottom reading, automatic focus adjustment, apertures, dichroic beam splitter selection, and fibreless optics ensure that there are no compromises in sensitivity and performance.

For demanding luminescence assays, the optional separate low noise detector gives extreme sensitivity. The microplate detector ensures optimal geometry with minimal crosstalk.

With true photon counting electronics for luminescence, time-resolved fluorescence, and AlphaScreen for greatest sensitivity, the Hidex Sense is officially certified for CisBio HTRF® TR-FRET, THUNDER™ TR-FRET, as well as Promega Dual-Luciferase® Reporter Assay Systems.

Unveiling the regulatory network controlling natural transformation in lactococci

In a study by Frédéric Toussaint et al. at the Louvain Institute of Biomolecular Science in conjunction with IFF Health & Biosciences, the Hidex Sense multimode compact plate reader has been used to identify the physiological conditions that activate competence for DNA transformation in Lactococcus lactis, the most important lactococcal species.

The microplate reader was used to monitor the expression of the ComX gene through luminescence. Growth (OD600) and luciferase activity were monitored at 5-minute intervals on the reader for a maximum of 24 hours.

You can learn more about the study and how the Hidex Sense was used by clicking the button below.

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Researchers from the Diabetes and Metabolism Group at Bristol Medical School chose the Hidex Sense multi-mode microplate reader to increase the throughput of luminescence assays as the group’s existing luminometer did not have stacking ability. The group were looking to match their current instruments impressive signal to background ratio for luminescence and therefore, the group chose the Hidex Sense. It ticked all boxes whilst offering additional capabilities such as TRF, absorbance and LSC which was useful for the group.

The group has been using the Hidex Sense microplate reader for assays using Nano-Glo® Luciferase. Nano-Glo® Luciferase assays allow researchers to detect the activities of firefly and luciferases in a single sample.

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Congratulations to Jussi Hepojoki and his colleagues at the Department of Virology, the University of Helsinki for their excellent work on the development of a rapid PCR test for Covid-19 that uses a TR-FRET assay for SARS-CoV-2 performed on the Hidex Sense Multi-Mode Microplate Reader.  

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Jordan Pascoe is a first-year PhD student in the Faculty of Health and Medical Sciences at the University of Surrey. Jordan has been using the Hidex Sense multi-mode microplate reader to study antimicrobial resistance and how the metabolic state of bacteria may affect treatment efficacy, as part of wider research into tuberculosis.

By utilising filter technology for fluorescence, the Sense delivers optimal sensitivity and faster reading speeds than other monochromator-based microplate readers. It also features a spectrograph that can scan samples from 220-1000nm in less than a second, without compromising on sensitivity.

We spoke to Jordan to find out how he’s been using the Sense in his research.

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Debbie Fischer and the Division of Pharmacy and Optometry at the University of Manchester are the new owners of the Hidex Sense microplate reader. Debbie is part of Kay Marshall’s research group investigating diagnostic biomarkers and improved therapies for female reproductive disorders.

Click the button below to find out what Debbie had to say. 

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Dr Brian Robertson, from Imperial College London’s Department of Medicine, is conducting vital research into creating a new vaccine for Tuberculosis (TB) using the Hidex Sense multi-mode microplate reader. The work is contributing towards the End TB Strategy from the World Health Organization (WHO).

Dr Brian Robertson is also a founding member of the MRC Centre for Molecular Bacteriology and Infection, and the Centre for Integrative Systems Biology and Bioinformatics. He is Co Editor-in-Chief of the journal Tuberculosis, and Director of the Wellcome Trust funded 4-year PhD programme in the Molecular and Cellular Basis of Infection.

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The latest adopter of the Hidex Sense multi-mode microplate reader is Dan Bose, a Henry Dale Research Fellow at the University of Sheffield. Dan is part of the Sheffield Institute for Nucleonic Acids and the Department of Molecular Biology and Biotechnology, and his research focuses on the role of non-coding RNA’s in the cell and how they regulate gene expression. 

The research in Dan’s department spans a range of disciplines, incorporating different aspects of biochemistry, molecular experimentation, to cell-based assays, and CRISPR gene editing. 

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The Hidex Sense multi-mode microplate reader is a compact, state-of-the-art instrument which maximises efficiency in your lab. It provides a powerful and versatile platform for performing any kind of assay across various application areas.

LabLogic recently caught up with the first ever UK customer, Dr Charles Brearley, from the University of East Anglia (UEA), to find out what he thought about the system.

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Veteran Affairs Medical Center's (VAMCs) throughout the USA are fast selecting the Hidex range as their research instruments of choice including Cleveland, OH, Little Rock, AR, and San Juan, PR, read more here.

A research group at the University of Leicester has cited the Hidex Sense in several research papers on their successful development of novel formats of ''pseudo''-ELISA assays using nanoMIPs on the instrument. Read more about the paper here.

The Brookhaven National Laboratory has carried out a series of experiments using the Hidex Sense. Read more about the three-week long analysis here.